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Introduction of Genomics Lectures

Nazwa przedmiotu: Wstęp do genomiki, transkryptomiki i proteomiki (Introduction to genomics, transcriptomics and proteomics) [0501-IGTP-DX]
Typ Przedmiotu: Przedmiot do wyboru.
Rok Studiów, kierunek, semestr: III lic oraz I, II, mgr
Liczba punktów, ECTS: 30 h Pkt ECTS 3/Z
Spis zalecanych lektur: Podręcznik: Genome transcriptome and proteome analysis: By Alain Bernot; Wiley Publisher, 2004.
Treści merytoryczne przedmiotu:
1. Review of molecular genetics: Nucleic acids, constituents of the genome, organelle genomes, gene structure, transcription and translation, genes and multiple gene families and conserved sequences, coding and non-coding fractions of the genome.
2. Tools of molecular biology: restriction enzymes, electrophoresis, cloning and constructions of library, hybridization techniques, enzymatic amplification, chromosomal assignment, techniques specific to genome analysis.
3. Specifics of genome program: creation of the genome programs, genome research centres, growing importance of bioinformatics, automation and robotics.
4. Species genome analysis: the main branch of life, prokaryotes, eukaryotes
5. Linakge maps: tools and methods in genetic mappings, development of genetic maps, radiation hybrid maps
6. Physical maps: local maps or small genomes,
7. Strategies for physical mapping of the human genome, maps of the human genome.
8. Genome sequencing: strategies choices, prokaryotic genome, genome of model eukaryotes, the human genome, other genome sequenced
9. Sequencing cDNA and transcriptome: strategies of cDNA sequencing, the economic stakes, the analysis of cDNA sequences, the transcriptome
10. The proteome: basic techniques
11. Transgenesis
12. Mutagenesis
13. Two dimensional electrophoresis and identification of protein
14. Identification of protein interactions by two hybrid system
15. Chip technology
16. Analysis of three dimensional structure
17. Identification of gene responsible for the disease: genetic diseases
18. Functional cloning and chromosomal anomalies
19. Strategies for positional cloning
20. What is the future for the cloning of disease causing genes